Thanks to the gentle effect of the water-jet, the surrounding tissue, nerves and vascular structures remain virtually undamaged. This minimizes the risks involved with liposuction, improves the result and shortens the patient\u2019s recovery.<\/p>\n Likewise, the FJU includes the bones, cartilage, muscles, ligaments, tendons, vasculature etc. of the joint.<\/p>\n Local nerve pain (neuropathy) is also addressed by injecting on the sides of the neurovascular bundles (perineural) under ultrasound guidance.<\/p>\n Biodistribution and anatomical studies have also established the lymphatics vessels\u2014which parallel the vascular system\u2014as a route that stem cells may migrate through to bypass the blood-brain-barrier.[3]<\/a> [4]<\/a><\/p>\n [\/et_pb_text][et_pb_text _builder_version=”3.22.4″ custom_padding=”0px|||||” global_module=”7842″]<\/p>\n References:<\/strong><\/p>\n [\/et_pb_text][et_pb_text _builder_version=”3.22.5″ text_font_size=”80%” text_line_height=”1.5em”]<\/p>\n [1]<\/a> M Tobita et al Adipose tissue-derived mesenchymal stem cells and platelet-rich plasma: stem cell transplantation methods that enhance stemness Stem Cell Research & Therapy (2015) 6:215<\/p>\n [2]<\/a> \u00a0C.V. Borlongan et al Permeating the Blood Brain Barrier and Abrogating the Inflammation in Stroke: Implications for Stroke Therapy Curr Pharm Des. 2012; 18(25): 3670\u20133676.<\/p>\n [3]<\/a> J. Leibacher and R. Henschler Biodistribution, migration and homing of systemically applied mesenchymal stem\/stromal cells Stem Cell Therapy and Research 2016; 7: 7.<\/p>\n [4]<\/a> M. Absthina et al Human and nonhuman primate meninges harbor lymphatic vessels that can be visualized noninvasively by MRI eLife 2017;6: e 29738.<\/p>\n [\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ admin_label=”Footer CTA” _builder_version=”3.22.4″ background_image=”https:\/\/ambrosecelltherapy.com\/wp-content\/uploads\/2018\/12\/Cover-Revision-e1544054347204.jpg” background_blend=”multiply” custom_padding=”25px|0px|25px|0px|true|false” animation_style=”zoom” animation_intensity_zoom=”4%” global_module=”317″][et_pb_row custom_padding=”|||” parallax_2=”on” _builder_version=”3.22.3″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” background_blend=”multiply” max_width=”80%” animation_style=”slide” animation_direction=”top” animation_intensity_slide=”3%” use_custom_width=”on” width_unit=”off”][et_pb_column type=”1_2″ _builder_version=”3.0.47″][et_pb_text _builder_version=”3.18.4″ text_font=”Montserrat|700||on|||||” text_text_color=”#ffffff” text_letter_spacing=”3px” header_font=”|700|||||||” header_font_size=”36px” background_layout=”dark” custom_margin=”|||” animation_style=”slide” animation_direction=”bottom” locked=”off”]<\/p>\n The power of science, technology and your own biology.<\/p>\n [\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”3.0.47″][et_pb_button button_url=”https:\/\/ambrosecelltherapy.com\/contact\/” button_text=”Learn More” _builder_version=”3.19″ custom_button=”on” button_text_color=”#ffffff” button_border_width=”3px” button_border_color=”#ffffff” button_border_radius=”9px” button_letter_spacing=”2px” button_font=”Montserrat|on||on|” button_icon=”%%36%%” animation_style=”slide” button_text_color_hover=”#ffffff” button_border_color_hover=”#ffffff” button_letter_spacing_hover=”2px” button_bg_color_hover=”rgba(0,0,0,0)” saved_tabs=”all” locked=”off” button_text_size__hover_enabled=”off” button_text_size__hover=”null” button_one_text_size__hover_enabled=”off” button_one_text_size__hover=”null” button_two_text_size__hover_enabled=”off” button_two_text_size__hover=”null” button_text_color__hover_enabled=”on” button_text_color__hover=”#ffffff” button_one_text_color__hover_enabled=”off” button_one_text_color__hover=”null” button_two_text_color__hover_enabled=”off” button_two_text_color__hover=”null” button_border_width__hover_enabled=”off” button_border_width__hover=”null” button_one_border_width__hover_enabled=”off” button_one_border_width__hover=”null” button_two_border_width__hover_enabled=”off” button_two_border_width__hover=”null” button_border_color__hover_enabled=”on” button_border_color__hover=”#ffffff” button_one_border_color__hover_enabled=”off” button_one_border_color__hover=”null” button_two_border_color__hover_enabled=”off” button_two_border_color__hover=”null” button_border_radius__hover_enabled=”off” button_border_radius__hover=”null” button_one_border_radius__hover_enabled=”off” button_one_border_radius__hover=”null” button_two_border_radius__hover_enabled=”off” button_two_border_radius__hover=”null” button_letter_spacing__hover_enabled=”on” button_letter_spacing__hover=”2px” button_one_letter_spacing__hover_enabled=”off” button_one_letter_spacing__hover=”null” button_two_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover=”null” button_bg_color__hover_enabled=”on” button_bg_color__hover=”rgba(0,0,0,0.21)” button_one_bg_color__hover_enabled=”off” button_one_bg_color__hover=”null” button_two_bg_color__hover_enabled=”off” button_two_bg_color__hover=”null”][\/et_pb_button][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" The AMBROSE approach and cycle of care is personalized towards each patient\u2019s goals of improved function, symptoms and quality of life. AMBROSE Cell Therapy is a same-day, outpatient procedure which includes tissue harvesting, cell preparation and a cell delivery strategy uniquely developed with the patient\u2019s goals in mind. Step 1 All AMBROSE patient treatment plans […]<\/p>\n","protected":false},"author":8,"featured_media":379,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"[mk_page_section bg_image=\"https:\/\/ambrosecelltherapy.com\/wp-content\/uploads\/2018\/10\/contact-bg.jpg\" bg_position=\"center bottom\" bg_repeat=\"no-repeat\" bg_stretch=\"true\" enable_3d=\"true\" min_height=\"200\" js_vertical_centered=\"true\" sidebar=\"sidebar-1\"][vc_column css=\".vc_custom_1539721096205{padding-left: 80px !important;}\"][vc_empty_space][mk_fancy_title color=\"#ffffff\" size=\"28\" force_font_size=\"true\" size_phone=\"20\" line_height=\"50\" font_weight=\"400\" txt_transform=\"capitalize\" letter_spacing=\"1\" margin_bottom=\"0\" font_family=\"none\"]\r\n![\"Water](\"https:\/\/ambrosecelltherapy.com\/wp-content\/uploads\/2018\/12\/Body-Jet-1.png\")
<\/a>All AMBROSE patient treatment plans begin with a mini-liposuction (approximately 20 ounces), using an innovative\u00a0water-jet fat harvesting technology. Fat cells are gently detached from the connective tissue by means of a fan-shaped pulsating water jet and simultaneous aspiration.<\/p>\nStep 2<\/strong><\/h3>\n
<\/a>Approximately 15 ounces of lipoaspirate are processed in the Celution\u2122<\/sup><\/strong> System in a little over two hours. Celution is approved in over 40 countries internationally as well as for 9 ongoing or completed FDA-approved trials. Over 25 human studies using Celution-processed ADRCs have been reported in the medical literature for a variety of conditions including chronic wounds, post-cancer treatment side effects, congestive heart failure (CHF), cirrhosis of the liver, erectile dysfunction and others.<\/p>\nStep 3<\/strong><\/h3>\n
<\/a>The remaining lipoaspirate (approximately 5 ounces), is \u201cpurified\u201d through the PureGraft\u2122<\/strong> filtration system. PureGraft removes red blood cells, lipids from damaged adipocytes and debris from the lipoaspirate. Next, 120 ccs of blood are drawn and spun in the Emcyte Pure PRP<\/strong>\u2122 system, a high concentration platelet rich plasma (PRP). Due to its stimulating effect on stem cells, PRP is mixed with the purified fat before direct injections. The primary goal of enriching the purified fat with PRP is to get these powerful cells to work harder and faster.[1]<\/a><\/p>\nStep 4<\/strong><\/h3>\n
<\/a>While the Celution system liberates the ADRCs from the lipoaspirate, the PRP-enriched Pure Fat is injected under real-time ultrasound (US) guidance into the Functional Spine Unit (FSU) <\/strong>and\/or the Functional Joint Unit (FJU). <\/strong>\u00a0The FSU is a complex anatomy including the bones, discs, joints, muscles, tendons, nerves, ligaments and vasculature that comprise and support our spine.<\/p>\n<\/a>Under ultrasound guidance, the AMBROSE medical team visually confirms and addresses with direct injections, each structure that contributes to pain and lost function. Each has a connection to the others and provides a comprehensive approach to rescue the entire FSU or FJU unit.<\/p>\nNeuropathies<\/strong><\/h3>\n
Step 5<\/strong><\/h3>\n
<\/a>Finally, your ADRCs are delivered at the bedside via intravenous (IV) infusion. When a patient has a neurologic complaint, a low dose of mannitol (a sugar alcohol) is delivered IV prior to the stem cell infusion. Mannitol temporarily opens up the blood brain barrier so that a significantly higher percentage of cells can safely migrate into the brain to address the neuroinflammation and cascade of degenerative effects that can result from it.[2]<\/sup><\/a><\/p>\n<\/a><\/p>\nContinuing the Cycle of Care<\/strong><\/h3>\n
<\/a>Following a period of observation and recovery, patients return home to rest with a caregiver or family member. In the weeks and months that follow, the AMBROSE cycle of care continues with regular follow-up and health coaching geared towards helping our patients optimize lifestyle habits which support maximum benefit from cell therapy.<\/p>\nAMBROSE Cell Therapy<\/strong><\/h2>\n
Our Approach<\/span><\/h2>\r\n
<\/h2>\r\n[\/mk_fancy_title][\/vc_column][\/mk_page_section][vc_row][vc_column width=\"1\/6\"][\/vc_column][vc_column width=\"2\/3\"][mk_padding_divider][vc_column_text css=\".vc_custom_1543613238444{margin-bottom: 0px !important;}\"]The AMBROSE approach and cycle of care is personalized towards each patient\u2019s goals of improved function, symptoms and quality of life. AMBROSE Cell Therapy care plans each consist of tissue harvesting, cell preparation and a cell delivery strategy uniquely developed with the patient\u2019s goals in mind. This outpatient, same-day procedure consists of three key phases:\r\n
Phase 1: Tissue Harvesting<\/strong><\/h3>\r\nAll AMBROSE care plans begin with a fat harvesting procedure, performed by a board certified plastic surgeon, utilizing body-jet\u00ae water-assisted liposuction technology. Using this innovative system ensures minimal trauma for the patient and both a tissue- and cell-friendly harvest.\r\n\r\nWater-assisted liposuction differs greatly from conventional liposuction methods. A tissue harvest done using the body-jet\u00ae system gently detaches fat from the tissue structure with the help of a pulsating water jet. The result is minimized risk of bruising and swelling, shorter convalescence and heightened patient satisfaction with the secondary cosmetic outcome.\r\n\r\n
<\/a>Most importantly, surrounding tissue, nerves and vascular structures sustain much less damage with the body-jet\u00ae system. Sparing tissue and avoiding extensive trauma is highly relevant to achieving and even enhancing the therapeutic outcome of cell therapy.\r\nPhase 2a: Filtration and Enrichment <\/strong><\/h3>\r\nFollowing tissue harvest, the fat is further cleaned and filtered of debris using the FDA-approved PureGraft\u2122 system.\r\n\r\n
<\/a>The purified fat is then enriched with platelet rich plasma (PRP) to release growth factors, which are various proteins that promote the growth, organization and maintenance of cells and tissues.[i]<\/sup><\/a> PRP has a stimulating effect on the stem cells in fat and improves their retention so they work harder and longer. [ii]<\/a> [iii]<\/a>\r\n\r\n[Need new illustration of PRP here.]<\/strong>\r\nPhase 2b: Ultrasound Guided Direct Injections<\/strong><\/h3>\r\n
<\/a>Using ultrasound guidance at the point of care for a 3D view of the anatomy, direct injection sites are mapped out. The purified and enriched fat is then delivered to the target sites according to the individualized care plan and using specific injection protocols documented for each compartment in the joints, muscles, ligaments, tendons and nerve bundles of the musculoskeletal system.[iv]<\/sup><\/a>\r\nPhase 3a: Cell Processing and Separation<\/strong><\/h3>\r\nAll AMBROSE care plans include the delivery of adipose-derived stem and regenerative cells (ADRCs) via IV infusion, a step which calls for the use of the closed, sterile Celution\u00ae System for cell processing.\r\n\r\nIntegrated into the Celution\u00ae process and protocol is the use of a non-mammalian based enzyme which digests the adipose tissue and liberates the ADRCs. A second enzyme is also used which separates the cells from one another and prevents clumping.\r\n\r\nFinally, the resultant cell product is triple-washed to remove any residual enzyme or other debris. With the above complete, the ADRC preparation is ready for IV infusion or direct injection if called for in the treatment plan.\u00a0<\/strong>\r\n
Phase 3b: Permeating the Blood-Brain Barrier<\/strong><\/h3>\r\nIn certain cases, a low dose of mannitol is delivered intravenously prior to the stem cell infusion. Mannitol is a sugar alcohol which temporarily opens up the blood brain barrier so that a significantly higher percentage of cells can safely migrate into the brain.[v]<\/sup><\/a>\r\n
Phase 3c: IV Infusion <\/strong><\/h3>\r\nBecause adult stem cells \u201chome\u201d to sites of inflammation, chronic conditions with localized or low-level systemic inflammation can be safely addressed with intravenous (IV) administration. An infusion of ADRCs in this manner can address underlying degeneration and reinforce the process of repair.\r\n\r\nThe ADRC preparation is diluted in 100 CCs of saline and is released through the vein in an arm over a 20 minute period. Patients can rest comfortably in a recovery room while this final step takes place.\r\n
Aftercare and Follow-up <\/strong><\/h3>\r\nFollowing a period of observation and recovery, patients return home to rest with a caregiver or family member. In the weeks and months that follow, the AMBROSE cycle of care continues with regular follow-up and health coaching geared towards helping our patients optimize lifestyle habits which support maximum benefit from cell therapy.\r\n\r\n
\r\n\r\nImprove your quality of life.<\/span><\/h2>\r\n[\/mk_fancy_title][vc_column_text disable_pattern=\"false\" css=\".vc_custom_1539721050441{margin-bottom: 0px !important;}\"]Reverse the degenerative process and start feeling better.[\/vc_column_text][\/vc_column][vc_column width=\"1\/2\"][mk_button dimension=\"outline\" corner_style=\"full_rounded\" size=\"medium\" letter_spacing=\"1\" url=\"https:\/\/ambrosecelltherapy.com\/contact-us\/\" align=\"center\" margin_bottom=\"20\" margin_right=\"0\" outline_skin=\"light\"]CONTACT US >[\/mk_button][\/vc_column][\/mk_page_section]","_et_gb_content_width":"","footnotes":""},"class_list":["post-240","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\n